Purification and characterization of P fimbriae from an Escherichia coli strain isolated from a septicemic turkey.

نویسندگان

  • S A Pourbakhsh
  • J M Fairbrother
چکیده

A pap+ Escherichia coli isolate from a turkey with colisepticemia expressed P fimbriae with a major subunit of an apparent molecular mass of 18 kDa which reacted with anti-F11 serum. This fimbriae was purified and polyclonal antiserum was produced in rabbits. The N-terminal amino acid sequence of the major fimbrial subunit of the avian P fimbriae was identical to that of F11. On immunoblotting, the antiserum against the avian P fimbriae strongly reacted with the major subunit of the homologous fimbriae, with F11, and with F165(1) fimbriae. Some antigenic determinants on the major subunits of F13, F7(1), and F7(2) fimbriae, with a stronger reaction against F13 fimbriae, were also recognized. The F11 antiserum reacted similarly to the antiserum against avian P fimbriae although cross-reactions against F13, F7(1), and F7(2) fimbriae were equivalent. In a competitive enzyme-linked immunosorbent assay, serological differences were observed between the purified avian P fimbriae and F11. Thus, the avian P fimbriae is closely related but not identical to F11 fimbriae which are associated with E. coli isolated from human urinary tract infection.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Production, Purification and Characterization of Chicken Egg Yolk Monoclonal Antibody Against Colonization factor antigen -1 of Enterotoxigenic Escherichia coli Causing Diarrhea

Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in both humans and animals. The contaminated food and water are the most common vehicles for ETEC infection. The colonization factor antigen (CFA-1) is a fimbriae protein that promotes adherence of the ETEC strain to the epithelium of the small intestine of the host. In this study IgY proteins were produced against the CFA-1 of ETEC in imm...

متن کامل

Construction of a Nanobodies Phage Display Library From an Escherichia coli Immunized Dromedary

Background: Diarrhea caused by Escherichia coli is a major cause of morbidity and mortality in young animals. Few treatment options are available, mainly antibiotic therapy increasingly limited by resistance to commonly used drugs.Objectives: The aim of this work was to develop immunotherapy based on the use of camel VHH antibody fragments, or nanobodies,...

متن کامل

Molecular Study of Phase Variation of Type 1 Fimbriae in Uropathogenic Escherichia coli O44 Serotypes during Touching with Solid Surfaces

Background & Aims: Type 1 fimbriae is the most common adhesion factor in urine tract infection. In this Study, presence of virulence genes in isolated strains of uropathogenic E.Coli, O serotyping and molecular detection of phase variation of type 1 fimbriae were assessed during solid surfaces exposure. Methods: Isolated E.coli from urine samples of patients were serotyped by using serologic me...

متن کامل

بررسی فراوانی سروتایپ‌های انتروپاتوژن دارای ژن‌ pap G در اشریشیاکلی جداسازی شده از ادرار و خون بیماران بستری در بیمارستان‌های شهرستان خرم‌آباد

Background and Objective: Escherichia coli (E-coli) is a normal gram negative organism that inhabit the human and animal intestinal tract. Pap G gene has an important role in creating P -fimbriae tip and in producing septicemia and urinary tract infection. The aim of this study was to investigate the presence of pap G gene in EPEC strains isolated from urine and blood. Materials and Metho...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • FEMS microbiology letters

دوره 122 3  شماره 

صفحات  -

تاریخ انتشار 1994